This project investigates how general inflammatory conditions influence the transition from pre-disease to disease states in autoimmune and allergic disorders. Environmental factors such as obesity, hypoxia, or diet can induce systemic inflammation, which may convert non-pathogenic immune responses into pathogenic ones. A key biomarker of such inflammatory immune states is total serum IgG Fc glycosylation, where reduced sialylation reflects increased inflammation. Building on the Ehlers group’s expertise in antibody glycosylation, this study will combine high-performance liquid chromatography (HPLC) for serum IgG Fc glycosylation analysis, flow cytometry for immune cell profiling and α-2,6- sialyltransferase expression, and nuclear magnetic resonance (NMR) spectroscopy for lipidomic markers. These methods will be applied to samples from healthy individuals and patients with inflammatory conditions such as sleep apnoea, allergy, and autoimmune diseases before and after treatment. By correlating immune and metabolic parameters, this project aims to define characteristic marker patterns distinguishing pre-inflammatory from inflammatory states. The results will contribute to identifying key mechanisms that drive the onset or remission of autoimmune and allergic diseases and may provide biomarkers for monitoring personal inflammatory status.