MD A7: Identification of autoantibodies contributing to the break of immunotolerance in immunization induced mucous membrane pemphigus mouse model
Mucosal pemphigoid (MMP) is one of the bladder-forming autoimmune diseases with predominant involvement of the mucous membranes. Autoantibodies target components of the basal membrane. In particular, it shows an activity directed against the protein laminin-332 which is associated with an increased risk of developing malignant tumours.Patients with MMP have been shown to have an HLA polymorphism whose influence on the development of the disease is still unclear. Tu study this mechanism, samples from the established MMP mouse model designed for anti-laminin 332 MMP are used. This shows great similarity to characteristics of human disease expression.Samples of this mouse model are used to determine the genetic impact on disease development, as well as the influence of antibody subclasses. To a certain extent, conclusions can be drawn about the underlying pathophysiology, in particular the development of antibodies against different epitopes or proteins.To achieve these objectives, samples in the form of serums and biopsies are collected from mice with three different genetic backgrounds. These were immunized with two different fragments of the murine laminin-332 alpha chain.Epitopes and proteins that can act as auto antigens in MMP are analyzed with the help of western blot and immunoprecipitation.In order to investigate the question of epitope spreading, nearby structural proteins (mCOL7, mCOL17) are included in the study. The autoantibodies are analyzed by ELISA regarding their subclasses. This examination is continued in the form of indirect and direct immunofluorescence.
- Projects
- 1st Generation
- A: Defining Autoimmune Pre-Disease
- B: Targeting of Autoimmune Pre-Disease
- Associated projects
- Medical doctoral researcher projects
- Concluded projects
- Doctoral researchers
- Medical doctoral researchers
- MD A1: Investigation of the influence of specific CDK inhibitors on neutrophil activation
- MD A2: Anatomical expression of target antigens in autoimmune blistering dermatoses as markers for lesion formation
- MD A3: Structural characterization of skin-directed autoantibodies and their interaction with the antigen to gain insights into autoimmune pre-disease
- MD A4: Do interactions between AT1R autoantibodies derived from patients with systemic sclerosis and endothelial cells lead to endothelial dysfunction?
- MD A5: Establishing a human 3D skin model for pemphigus vulgaris
- MD A6: Assessing vasculopathy in systemic sclerosis using optical coherence tomography
- MD A7: Identification of autoantibodies contributing to the break of immunotolerance in immunization induced mucous membrane pemphigus mouse model
- MD A8: Impact of angiotensin II type 1 receptor antibodies on endothelial dysfunction in systemic sclerosis
- MD A9: Impact of glycosylation on IgG4-induced signaling in neutrophils
- MD A10: Testing a new single chain variable fragment for pemphigus foliaceus in the human skin organ culture model
- MD A11: Impact of glycosylation on IgG3-induced signaling in neutrophiles
- MD A12: Screening for inhibitors to prevent keratinocyte dissociation
- MD A13: Investigation of the local and systemic complement activation in bullous pemphigoid patients
- MD A14: Impact of different IgG subclasses and glycosylation patterns on immune complex-induced signaling in neutrophils
- MD A15: Novel target antigens of the lower basal membrane zone as inducers of autoimmunity of bullous autoimmune dermatoses
- MD A16: Identification of the major epitope of the BP180 ectodomain recognized by serum IgA autoantibodies of patients with pemphigoid diseases – IgA autoantibodies as prognostic marker?
- MD A17: Autoantibody-mediated effects on endothelial and immune cell signaling in systemic sclerosis
- MD A18: Molecular and cellular characterization of pre-autoimmune effects induced by aging in mice
- MD A19: Immunogenic effects of Staphylococcus aureus toxins in autoimmune vasculitis
- MD B1: Testing the effect of kinase inhibitors in the human skin organ culture model for pemphigus vulgaris
- MD B2: Investigation of cigarette smoking-induced autoantibodies against human airway epithelial cells in patients with chronic obstructive lung disease
- MD B3: Contribution of taurine, pyridoxine and pantothenic acid to the pathomechanism of pemphigus vulgaris
- MD B4: The influence of prednisolone treatment on split formation in the human skin organ culture model for pemphigus vulgaris
- MD B5: Molecular characterization of the pre-autoimmune effects of Western diet in healthy mice
- MD B6: Testing established MAP kinase inhibitors in a different approach of the human skin organ culture model for pemphigus vulgaris
- MD B7: Testing the effect of kinase inhibitors in the human skin organ culture model for pemphigus foliaceus
- Ass. doctoral researchers
- Ass. medical doctoral researchers
- 2nd Generation
- 1st Generation
Medical doctoral researcher
Participating Researchers
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